RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell
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RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell
Gene expression studies by quantitative reverse-transcription PCR (qRT-PCR) can be challenging when attempted with samples of a few cells or a single cell. Epicentre’s MessageBOOSTERTM cDNA Synthesis from Cell Lysates kit enables RNA amplification and cDNA synthesis directly from cell lysates. A MessageBOOSTER kit reaction from a single cell produces enough cDNA for thousands of qPCRs, even wit...
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1The Rheumatology Section, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104; 2The Pediatric Rheumatology Section, The Childrens Hospital of Philadelphia, Philadelphia, Pennsylvania 19104; 3The Biotechnology Center, The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104 DNA amplification utilizing the polymerase chain ...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2009
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.280